The present invention provides a method of quantification nucleic acid sequences with minimal alterations conducting a real time PCR quantification assay with allele-specific primers. Further the present invention provides the method for quantifying allelic differences of bovine mitochondrial DNA sequences and allele-specific primers to be used in said method.
申请公布号
WO9940219(A1)
申请公布日期
1999.08.12
申请号
WO1999EP00648
申请日期
1999.02.02
申请人
BAVARIAN NORDIC RESEARCH INSTITUTE A/S;STEINBORN, RALF;MUELLER, MATHIAS;BREM, GOTTFRIED;KLEIN, DIETER