发明名称 |
Autonomously replicating sequences, GAPDH gene and promoter derived from Hansenula polymorpha, expression vectors containing same and method for the selection of transformants |
摘要 |
Autonomously replicating sequences(ARS), glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene and GAPDH promoter derived from Hansenula polymorpha DL-1(ATCC 26012); a vector for H. polymorpha which contains the novel ARS and is capable of inserting tandem repeating multiple copies of a polynucleotide encoding a foreign protein to the chromosome of H. polymorpha; a process for the production of a foreign protein in H. polymorpha by employing said vector; and a method for the selection of transformed H. polymorpha having multiple copies of integrated foreign genes.
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申请公布号 |
US5935789(A) |
申请公布日期 |
1999.08.10 |
申请号 |
US19970903800 |
申请日期 |
1997.07.31 |
申请人 |
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
RHEE, SANG-KI;CHOI, EUI-SUNG;KIM, CHUL-HO;SOHN, JUNG-HOON;KANG, HYUN-AH;KIM, HWA-YOUNG |
分类号 |
C07K14/485;C12N1/19;C12N9/02;C12N15/81;(IPC1-7):C12Q1/68;C12N9/72 |
主分类号 |
C07K14/485 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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