Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use

摘要

A nucleic acid amplification station for a test strip comprising a dual chamber reaction vessel comprising a first chamber and a second chamber, characterised in that the station comprises: a tray for at least one test strip, the said tray comprising a first portion and a second portion positioned adjacent to the said first and second chambers of the said dual chamber reaction vessel, respectively; a temperature control subsystem for the said tray maintaining the said first and second portions of the said tray at first and second different amplification reaction temperatures so as to maintain the said first and second chambers at the said first and second amplification reaction temperatures, respectively; a fluid conduit opening mechanism for opening a fluid conduit in the said dual chamber reaction vessel to establish fluid communication between the said first chamber and the said second chamber; and, optionally, a vacuum subsystem comprising a vacuum probe, the said test strip and vacuum probe being mutually reciprocable and the said vacuum probe cooperating with reaction wells in the said test strip for transferring a fluid sample from the said first chamber to the said second chamber via the said fluid conduit is disclosed.