OLIGOSACCHARIDES AND THE DERIVATIVES THEREOF, CHEMO-ENZYMATIC METHOD FOR THE PRODUCTION THEREOF

摘要

The invention relates to novel oligosaccharides and the derivatives thereof in addition to a general method for stereo divergent production of oligosaccharides from easily accessible simple glycosides, wherein a further saccharide element is stereo selectably created from the aglycone constituent thereof by means of chain elongation reactions. This is achieved by (optional) chemical addition of an aldehyde equivalent to a C=X-double bond in the aglycone, followed by diastereo-selective enzymatic addition of a nucleophile aldol donor to the glycosylated aldehyde in the presence of various stereo-specific aldolases. The resulting oligosaccharides, which carry an additional ketose unit on the reducing end when DHAP-dependent aldolases are used, and their corresponding phosphate esters and suitable derivatives thereof are useful as constituents of precursors for pharmaceutically active substances.