发明名称 PRODUCTION OF POLYPEPTIDE
摘要 PROBLEM TO BE SOLVED: To produce prokaryotic or eukaryotic cell protein by transforming a host with an expression vector that contains a specific DNA sequence as Escherichia coil phage T5, a DNA sequence coding a polypeptide and a specific DNA sequence in the downstream direction of the transcription and by culturing the transformer host. SOLUTION: A host is transformed with an expression vector including a DNA sequence of ribosome-bonding site-code-synthesis having, in the downstream direction of the transcription, Escherichia coli phage T5 or T7 promoter, a DNA sequence coding polypeptide, and a gram-positive microorganism expression control DNA having E. coli-originating T0 , T1 , T2 or T7 terminator.
申请公布号 JPH10229878(A) 申请公布日期 1998.09.02
申请号 JP19960185740 申请日期 1996.07.16
申请人 F HOFFMANN LA ROCHE AG 发明人 BUJARD HERMANN;LE GRICE STUART
分类号 C12N15/09;C12N1/20;C12N1/21;C12N9/04;C12N9/06;C12N9/10;C12N15/00;C12N15/70;C12N15/75;C12P21/02;C12R1/125;C12R1/19;C12R1/91 主分类号 C12N15/09
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