Method for the integration of a chosen gene into a bacterial chromosome, and bacterium obtained by said method

摘要

The invention relates to a method for the integration of a chosen gene or a specified DNA sequence into a DNA sequence such as a bacterial chromosome or episome, wherein: a) the chosen gene or chosen DNA sequence is cloned inside a transposon, stripped of its arms, outside the essential portions of the transposon, b) the transposon is integrated into the DNA sequence, such as the chromosome or episome of the bacterium, as well as to the bacterial strains obtained by carrying out this method.