发明名称 |
Compounds and methods for site directed mutations in eukaryotic cells |
摘要 |
The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single 3' and a single 5' end. These ends can be ligated so that the polynucleotide is a single continuous circular polymer. The polynucleotide can be used to induce specific alterations in targeted genes.
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申请公布号 |
US5565350(A) |
申请公布日期 |
1996.10.15 |
申请号 |
US19940353657 |
申请日期 |
1994.12.09 |
申请人 |
THOMAS JEFFERSON UNIVERSITY |
发明人 |
KMIEC, ERIC B. |
分类号 |
A01K67/027;C07H21/00;C07H21/02;C07H21/04;C07K14/82;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/80;C12N15/90;(IPC1-7):C12N15/63 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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