| 摘要 |
PURPOSE:To decompose ethylene chloride at high decomposition rate in high efficiency by using a transformant transformed with a recombination plasmid having high decomposition capacity of ethylene chloride such as trichloroethylene and tetrachloroethylene. CONSTITUTION:The recombination plasmid to be used in this process contains (A) a hybrid gene containing, as an active site, a gene fragment todC1bphA2A3A 4 produced by linking a partial gene todC1 of a structural gene coding a toluene- decomposition enzyme originated from Pseudomonas putida F1 to a partial gene bphA2A3A4 of a structural gene coding a biphenyl decomposition enzyme originated from Pseudomonas pseudoalcaligenes, (B) a drug-resistant gene and (C) a gene replicable in a Gramnegative bacteria. The plasmid is introduced into a host bacterium cell and the obtained transformant is used for the decomposition of ethylene chloride. |
