发明名称 |
USE OF BACTERIAL LUCIFERASE STRUCTURAL GENES FOR CLONING AND MONITORING GENE EXPRESSION IN MICROORGANISMS AND FOR TAGGING AND IDENTIFICATION OF GENETICALLY ENGINEERED ORGANISMS |
摘要 |
A host microorganism is genetically and stably modified by the insertion into any of its non-essential chromosomal location of a non-homologous, recombinant foreign DNA fragment, maintaining an insertion of a luxAB gene of a selected bioluminescent bacterium such as V.harveyi, such that the expression of the luxAB genes causes the production of a luciferase enzyme which, in turn, catalyzes a light-emitting reaction in the presence of the appropriate substrate. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism, each with a known and related cell (or plasmid) count.
|
申请公布号 |
CA1333776(C) |
申请公布日期 |
1995.01.03 |
申请号 |
CA19870542601 |
申请日期 |
1987.07.21 |
申请人 |
TEXAS A & M UNIVERSITY SYSTEM;BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH |
发明人 |
LEGOCKI, ROMAN P.;LEGOCKI, MISUK;SZALAY, ALADAR A.;BALDWIN, THOMAS O. |
分类号 |
C12N9/02;C12N15/90;C12Q1/06;C12Q1/68;(IPC1-7):C12Q1/68;C12N1/21;C12Q1/66;C12N15/74 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|