| 摘要 |
The present invention relates to DNA sequences encoding a rhamnogalacturonase which comprises (a) the DNA sequence of nucleotides 64-1587 of SEQ ID NO:1; (b) a DNA sequence which hybridizes to the same probe as nucleotides 64-1587 of SEQ ID NO:1 under conditions of presoaking in 5xSSC and prehybridizing for 1 hour at -40 DEG C. in a solution of 5xSSC, 5xDenhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 mg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 mu Ci 32-P-dCTP labelled probe for 18 h at -40 DEG C., followed by washing three times in 2xSSC, 0.2% SDS at 40 DEG C. for 30 minutes; or (c) a DNA sequence encoding an amino acid sequence having amino acids 20-527 of the sequence of SEQ ID NO;2. |
