| 摘要 |
A method for the simultaneous determination of cholesterol and triglyceride substrates with a single reagent system. The reagent system is reacted with the specimen such that each of the substrates reacts with their respective reactant simultaneously. The change in absorbance or fluorescence of the resulting reaction mixture is measured at a plurality of wavelengths which are characteristic for each of the substrates to be determined. The amount of cholesterol and triglyceride substrates can be determined by either an endpoint or reaction rate measurement. The reagent system comprises an enzyme having cholesterol esterase activity, a chromogenic oxygen acceptor, microperoxidase, and cholesterol oxidase for determination of cholesterol; and lipase, ATP, PEP, glycerol kinase, pyruvate kinase, LDH and NAD(P)H or analogs thereof for determination of triglyceride. |
