METHOD OF PRODUCING F(AB')2 FRAGMENTS OF IMMUNOGLOBULINS

摘要

In the field of monoclonal antibody production, the invention relates to the production of F(ab')2 and Fab' fragments from immunoglobulins. The fragments are produced by acidifying a hybridoma conditioned culture medium containing immunoglobulins with citric acid to a pH of about 3.5 and incubating the conditioned medium at 25 DEG C. A proteolytic enzyme in the conditioned medium cleaves the immunoglobulins to F(ab')2 fragments, which may then be treated with a reducing agent to generate Fab' fragments.