PRODUCTION OF DEACETYL-7-AMINOCEPHALOSPHORANIC ACID

摘要

PURPOSE:To rapidly obtain stable deacetyl-7-aminocephalosporanic acid by deacetylating the 7-aminocephalosporanic acid with a specific enzyme and employing ammonia as a neutalizing agent. CONSTITUTION:7-Aminocephalosporanic acid (7ACA) is treated with a 7ACA- deacetylating enzyme such as preferably cephalosporine acetylhydrolase originated from Bacillus subtilis preferably at a pH of 8.0-8.5 in a boric acid buffer solution at 20-22 deg.C and neutralized with ammonia water (in an amount sufficient for neutralizing the raw material 7ACA and by-produced acetic acid), followed by crystallizing the objective deacetyl-7-aminocephalosporanic acid.