| 摘要 |
PURPOSE:To provide the titled substance free from contamination of impurities, by culturing mutant of Bacillus subtilis capable of producing cyclodextrin glucanotransferase, and separating the cyclodextrin glucanotransferase from the culture product. CONSTITUTION:A chromosome DNA is prepared by Bacillus macerans IAM1243 strain. The chromosome DNA is partially decomposed with the restriction enzyme Sau3AI, and the chromosome DNA fragment of about >=2 Kb is separated from the decomposition product. Separately, phase lambdaD69 DNA is incised with the restriction enzyme BamH1, mixed with the above chromosome DNA fragment, and linked by the addition of T4DNA ligase to obtain the plamid pDS10. A mutant Bacillus subtilis is prepared therefrom. The Bacillus subtilis is cultured, and the objective cyclodextrin glucanotransferase is separated from the culture product. |
