Method for producing highly-active biologically active compounds immobilized on a carrier

摘要

A two-step process of immobilization of biologically efficient compounds is disclosed, in which the biologically efficient compounds are bonded from an aqueous solution by a hydrolytically stable linkage to a macroporous polymeric carrier containing reactive functional groups. In the first step, a hydrophobic adsorption occurs of the biologically efficient compounds on a macroporous matrix of the carrier, and an increase of their concentration on the solid surface. In the second step, the reaction takes place with the reactive functional groups of the carrier, and a covalent linkage is formed. The hydrophobic adsorption of compounds determined for immobilization is achieved by the addition of inorganic salts in a concentration from 0.5 to 3.0 mol/l. The invention can be utilized in the preparation of enzymatic catalysts for transformations of compounds and in the preparation of specific sorbents for both analytical and preparative affinity chromatography. These methods are employed in the fields of biotechnology, chemical and clinical analysis, food industry, pharmacology and clinical therapy.