| 摘要 |
PURPOSE:To produce biotin in high efficiency, by forming DNA to code gene information participating in biosynthesis reaction of biotin from desthiobiotin derived from a bacterium belonging to the genus Bacillus, capable of producing biotin. CONSTITUTION:A recombinant vector which is integrated with NDA to code gene information participating in biosynthesis reaction of biotin from destiobiotin derived from a bacterium belonging to the genus Bacillus, capable of producing biotin is formed. A bacterium which is transformed or transduced with the recombinant is cultivated in the presence of a procursor of biotin biosynthesis in a medium containing a proper carbon source to produce biotin, which is separated and purified. A recombinant vector which is integrated with DNA in the vicinity of a promoter under the control of promoter is preferable as the recombinant vector and a bacterium belonging to the genus Escherichia coli may be cited as the bacterium for transformation. |
