TEST DEVICE AND METHOD FOR IN A COLORED AQUEOUS FLUID BY DIFFUSE REFLECTANCE DETECTING A SOLUBLE ANALYTE MEASUREMENT

摘要

A test device of use in the diffuse reflectance colorimetric determination of analytes (e.g. glucose, ethanol) in coloured aqueous fluids (e.g. whole blood, blood serum, blood plasma) and its use in such determinations is disclosed. The test device comprises a diffuse reflective backing (12) to which is affixed a thin translucent hydrophilic substrate pad (11) (sample pad) which contains reagents (e.g. an oxydase for the analyte, a substance having peroxidase activity and a substance which will react with hydrogene peroxyde to give a coloured compound). The reagents react with the analyte being determined. The device finds preferred application in the measurement of sugars in fluids, especially glucose in whole blood. It requires only a small sample size and allows for simple and more accurate colorimetric determinations than before.