| 摘要 |
PURPOSE:A sealed container containing an isotonic solution in the interior is connected to a culture container which has a granular or powder medium in the interior and is kept in a reduced pressure state by a connecting tube equipped with communicating pieces, a test specimen is inoculated into the isotonic solution, blended with it and distributed to the culture containers in the closed state. CONSTITUTION:A test specimen is injected into the container 1, inoculated into the isotonic solution 2, blended with it, the head parts of the small pieces of the communicating pieces 16 are folded and the connecting tube 25 is opened. The blended solution in the container 1 is divided into approximately equal amounts in the containers 7 and 8. The containers 7 and 8 contain the powder and/or granular mediums 9 and 10 and are kept in a reduced pressure. The clamps 17 and 18 are pressed to close the tube 15 and the ventilation needle 19 is pierced into the thin part 12a of the rubber stopper of the aerobic culture container 8 to make the interior of the container 8 at normal pressure.
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