METHOD FOR DETECTING THE PRESENCE OF GD3 GANGLIOSIDE

摘要

Method of determining the presence of GD3 disialoganglioside in cell lines and tissues comprising reacting antibody R24 (AbR24) with a test sample of the cell lines and tissues and determining the extent of the reaction. Four assay methods are used:
(a) passive hemmaglutination
(b) antibody inhibition
(c) glycolipid-mediated immune adherence
(d) a method using <1><2><5>I-Protein A to detect AbR24 reacting with GD3 on thin layer chromatograms.
From (a) to (d) the sensitiveness of the assay methods is increased.