| 摘要 |
PURPOSE:Variant Escherichia coli useful for producing industrially heat-resistant alpha-amylase, by cloning a heat-resistant alpha-amylase structural gene of Bacillus stearothermophilus in a mold of Escherichia coli, so that heat-resistant alpha- mylase can be produced. CONSTITUTION:Chromosome DNA is separated from a culture mold of (Bacillus stearothermophilus) A631, IAM11003, ATCC7954 which is middle thermophilic bacterium to secrete thermophilic-amylase out of the mold, it is partially decomposed with restriction enzyme Sau3A, and a fregment of chromosome is separated. Separately, plasmid pBR322 is scissored with restriction enzyme BamHI, it is blended with the fragment of chromosome, ligase T4DNA is added to them, they are connected, the treated solution is added to suspension of culture mold of Escherichia coli C600, which is transformed to give the aimed variant Escherichia coli having plasmid pTUE107. |
