摘要 |
PURPOSE: To obtain the oils and fats effecitive for preventing and treating geriatric diseases having high diglyceride and monoglyceride contents and excellent digestion and absorption, containing docosahexaenoic acid in high concentration among constituent fatty acids of oils and fats. CONSTITUTION: A reaction solution comprising 0.05 M tris-hydrochloric acid buffer solution (pH 8.2), 0.2 M calcium chloride and 5% gum arabic is mixed with a lipase derived from Candida lipolytica and a fish oil collected from a head part of tuna, stirred at 45 deg.C for 15 hours and hydrolyzed while adjusting to pH 8.2 with 2 N NaOH. The formed decomposition oil is extracted with hexane and the extracted solution is stirred with acetone and then with 0.3 N sodium hydroxide solution at room temperature for 1 hour to provide the objective highly unsaturated fatty acid-containing fats and oils effective for preventing and treating geriatric diseases containing >=60% docosahexaenoic acid(DHA) among constituent fatty acids of fats and oils and >=80% based on fats and oils of total amount of diglyceride and monoglyceride. |