| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new virus vector comprising a partial or complete E3 region of wild type adenovirus 5 type and a gene capable of transducing and having therapeutic potential, capable of transducing human gene in an organism and useful for treatment, etc., of hemophilia. SOLUTION: This new adenovirus vector comprises a partial or complete E3 region of a wild type adenovirus 5 type and a gene capable of transacting and having therapeutic potential, e.g. a gene coding human coagulation factor IX and is used for gene transduction and is capable of transducing human gene in an organism and can be utilized for treatment, etc., of hemophilia by transduction of human coagulation factor. The adenovirus vector is obtained by preparing cDNA of human coagulation factor IX from whole RNA of human liver by a reverse transcriptase-liked polymerase chain reaction and binding the cDNA to wild type adenovirus complete E3 region, a cytome-galovirus promotor, a polylinker sequence, a termination signal and a restriction enzyme- cleaved site, etc. |
