PROCESS FOR PRODUCING PEPTIDE WITH THE USE OF ACCESSORY PEPTIDE

摘要

A process for producing a peptide having a desired biological activity via gene recombination characterized in that an accessory peptide is added to the target peptide followed by the expression thereof. Use of this process makes it possible to prevent agglutination in the recovery and purification steps with the regulation of isoelectric points, to achieve an elevated yield in ion exchange chromatography, to improve the solubility of a fused protein in an enzymatic reaction wherein the target peptide is expressed as the fused protein with another protein and then liberated by enzymatic cleavage, to improve the solubility of the target peptide in an enzymatic reaction wherein the peptide should be enzymatically modified, etc., thus elevating the purification efficiency and yield of the target protein. <IMAGE>