Selection of triploid CHO cells and cell lines expressing recombinant polypeptides as high producers

摘要

The present invention relates to a method for selecting for cells or cell lines that produce a recombinant protein/polypeptide in high yields, the method allowing for the selection of high producer cells or cell lines in an early phase of cell line development, the method comprising the step of determining the nuclear DNA content of the cells or cell lines, wherein the level of the nuclear DNA content of the cells or cell lines positively correlates with the capacity of the cells or cell lines to produce the recombinant protein/polypeptide.

主权项

1. A method of producing a Chinese Hamster Ovary (CHO) cell or a CHO cell line that expresses a recombinant protein/polypeptide in high yields comprising:
(a) transfecting a CHO cell or CHO cell line with a nucleic acid segment (i) encoding said recombinant protein/polypeptide, and (ii) providing for its expression in the CHO cell or CHO cell line; (b) measuring the nuclear DNA content of transfected CHO cells or cell lines; and (c) selecting a CHO cell clone or CHO cell line exhibiting a triploid nuclear DNA content, wherein the selected CHO cell clone or CHO cell line of step (c) expresses said recombinant protein/polypeptide at high yield.