主权项 |
1. An isolated nucleic acid, comprising:
a first nucleotide sequence encoding a signal peptide, and a second nucleotide sequence encoding a single chain antibody capable of forming an interface disulfide bond, the second nucleotide sequence being located 3′ downstream to the first nucleotide, wherein the signal peptide has the amino acid sequence of VKKLLFAIPLVVPFYX1X2X3X4X5X6X7X8X9X10HHHGH (SEQ ID NO:598), in which X1 is A, C, D, F, G, I, L, M, N, P, Q, R, S, T, V, or Y; X2 is A, C, D, F, G, H, K, L, N, P, Q, R, S, T, V, W, or Y; X3 is A, C, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, or Y; X4 is A, C, D, F, G, H, I, L, M, N, P, Q, R, S, T, V, W, or Y; X5 is A, C, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, W, or Y; X6 is A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, or Y; X7 is A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, or Y; X8 is A, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, or Y; X9 is A, D, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, or Y; and X10 is A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, or Y. |