发明名称 |
Muscle cell patches and uses therefor |
摘要 |
Disclosed herein are contractile cell constructs comprising contractile cells, or progenitors thereof, adhered to a surface of a three dimensional fibroblast containing scaffold (3DFCS) and methods for using them to treat disease. In one aspect, the present invention provides methods for preparing a contractile construct, comprising (a) seeding immature contractile cells onto the surface of a three dimensional fibroblast containing scaffold (3DFCS) to produce a contractile construct; and (b) culturing the contractile construct under conditions to promote differentiation of the immature contractile cells into mature contractile cells, wherein the mature contractile cells form striations. In a further aspect, the invention provides methods for treating a disorder characterized by a lack of functioning contractile cells, comprising contacting a patient with a contractile cell-based disorder with an amount effective to treat the disorder with the construct of any embodiment or combination of embodiments of the invention. |
申请公布号 |
US2016250384(A1) |
申请公布日期 |
2016.09.01 |
申请号 |
US201415028606 |
申请日期 |
2014.10.08 |
申请人 |
THE UNIVERSITY OF ARIZONA ;THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS |
发明人 |
LANCASTER Jordan J.;GOLDMAN Steven |
分类号 |
A61L27/38;C12N5/00;A61L27/36;C12N5/077;A61L27/58;A61L27/54 |
主分类号 |
A61L27/38 |
代理机构 |
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代理人 |
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主权项 |
1. A method for preparing a contractile construct, comprising
(a) seeding immature contractile cells onto the surface of a three dimensional fibroblast containing scaffold (3DFCS) to produce a contractile construct; and (b) culturing the contractile construct under conditions to promote differentiation of the immature contractile cells into mature contractile cells, wherein the mature contractile cells form striations. |
地址 |
Tucson AZ US |