HIGH PRESSURE SPERM SORTING AND FLOW CYTOMETER

摘要

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm sorting the steps of obtaining a sperm sample, staining sperm in the sperm sample with a DNA selective dye and a quenching dye in a single dilution, and sorting stained sperm in the sperm sample with a flow cytometer at an elevated pressure may be incorporated individually, or in combination to help sperm better survive the sex sorting process. WO 2014/ i5173PCVTUS20131063 284 [ 14 '34 *__ >30 6-'28 C)36 386 --- -- -- --- -- - ___ __ __ __ ___ __ __40 / FI ,6