发明名称 |
EFFICIENT DELIVERY OF LARGE CARGOS INTO CELLS ON A POROUS SUBSTRATE |
摘要 |
In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells. |
申请公布号 |
EP3122885(A1) |
申请公布日期 |
2017.02.01 |
申请号 |
EP20150769138 |
申请日期 |
2015.03.26 |
申请人 |
The Regents of the University of California |
发明人 |
WU, Ting-Hsiang;CHIOU, Pei-Yu, E.;TEITELL, Michael, A. |
分类号 |
C12N15/87;C12N1/15;C12N1/19;C12N5/071 |
主分类号 |
C12N15/87 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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