发明名称 |
PLASMID-ENCODED NEUROTOXIN GENES IN CLOSTRIDIUM BOTULINUM SEROTYPE A SUBTYPES |
摘要 |
The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique C. botulinum type A strains and encode either BoNT/ A3 or BoNT/ A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a C. botulinum type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/ A3 in the A3 strain, and BoNT/ A4 and BoNT/B in the A4 strain, are on plasmids. |
申请公布号 |
CA2691825(C) |
申请公布日期 |
2016.05.17 |
申请号 |
CA20082691825 |
申请日期 |
2008.06.27 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
JOHNSON, ERIC A.;MARSHALL, KRISTIN;PELLETT, SABINE;BRADSHAW, MARITE |
分类号 |
C12N15/74;C07K14/33;C12N1/20;C12N15/31;C12P21/02 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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