Cell programming

摘要

The present invention is concerned with methods for reprogramming of mammalian somatic cells and in particular to reprogramming of mature mammalian somatic cells into multi-potent precursor cells.

主权项

1. A method of reprogramming a mature mammalian fibroblast into a multi-potent neural precursor cell, said method comprising the steps of: a) delivering transcription factors consisting of Sox2 and Pax6 to said mature mammalian fibroblast via protein transduction or via protein expression from a transfected plasmid vector, wherein said transcription factors, in combination, induce conversion of said mature mammalian fibroblast into a multi-potent neural precursor cell; and b) culturing said mature mammalian fibroblast from step a) in medium supporting growth of said multi-potent neural precursor cell.