SEQUENCE OPTIMIZATION FOR EXPRESSION OF A FOREIGN GENE

摘要

The invention relates to methods useful for optimizing expression of a foreign gene in an organism or tissue of interest. In particular, the invention relates to methods for ranking genes expressed in an organism or tissue of interest and methods for modifying nucleotide sequences for particular uses by making changes in codon usage to modulate expression of a gene. In one aspect, the invention provides a method for optimizing a the nucleotide sequence for expression in a specific organism or tissue by recursively overlapping the nucleotide sequence of a target gene with the nucleotide sequences of ranked genes in a tissue of interest. In another aspect, the method provides for the construction of an optimized version of the target gene which retains the original amino acid coding information of the target gene.