PROMOTERS FOR HIGH LEVEL RECOMBINANT EXPRESSION IN FUNGAL HOST CELLS

摘要

The present invention provides, in part, promoters for recombinant expression of polypeptides in host cells such as Pichia pastoris as well as methods of use thereof. In one embodiment, an isolated hybrid polynucleotide comprises a promoter selected from a group of Pichia pastoris promoters. In another embodiment, a method for making a polypeptide by introducing an isolated hybrid polynucleotide into an isolated fungal host cell while utilizes a Pichia pastoris promoter.