Skin cream

摘要

The present invention relates to skin care compositions, including cosmeceuticals, for topical application, and more particularly, a skin cream, comprising exosomes and cell culture medium conditioned by cells grown in two-dimensional culture. Also included are methods of making and using such compositions and kits comprising the skin cream therein.

主权项

1. A skin cream for treating a skin defect, comprising exosome enriched conditioned medium from cultured substantially homogenous foreskin derived fibroblast cells, wherein said exosomes are isolated from cultures of cells grown under conditions to promote secretion of at least one growth factor into nutrient medium, wherein:
(i) the exosomes are isolated from conditioned media that is the same as the exosome enriched conditioned medium, which fibroblast cells are from a cell line designated as ATCC Accession No. PTA-11680 or are from a cell line designated as ATCC Accession No. PTA-11681; or (ii) the exosome enriched conditioned medium is generated from transformed cells and the exosomes are isolated from conditioned medium generated from non-transformed cells; or (iii) the exosome enriched conditioned medium is generated from non-transformed cells and the exosomes are isolated from conditioned medium generated from transformed cells, and wherein said transformed cells are from the cell line designated as ATCC Accession No. PTA-11680 and said non-transformed cells are from the cell line designated as ATCC Accession No. PTA-11681.