| 摘要 |
Techniques are disclosed relating to analysis and reduction of crosstalk between signals. These techniques may be applicable in many fields, such as single-tube PCR or DNA melt analysis, PCR or melt data from neighboring wells of a multi-well plate, capillary electrophoresis data (e.g., DNA sequencing), gas chromatography, multispectral imaging, dual-color fluorescence correlation spectrometry, electrical crosstalk, etc. According to one embodiment, crosstalk between fluorescence signals from different species may be determined based on a correlation between the time derivatives of the fluorescence signals from the fluorescent species. |
| 主权项 |
1. A method, comprising:
illuminating a sample with a light source, wherein the sample includes a plurality of fluorescent species, and wherein illuminating the sample with the light source excites at least two of the plurality of fluorescent species; measuring at least two fluorescence signals from the sample, wherein the at least two fluorescence signals include component signals from the at least two of the plurality of fluorescent species; and determining, by an assaying device, an amount of crosstalk between the at least two fluorescence signals, wherein the determined amount of crosstalk is based on a correlation between respective time derivatives of the at least two fluorescence signals. |
