摘要 |
<p>The present invention relates to a set consisting of four primers for an isothermal amplification reaction for detecting a watermelon mosaic virus, a composition comprising the same, and a method for detecting watermelon mosaic virus using the composition. The detecting method according to the present invention uses the isothermal amplification method to effectively detect all seven kinds of mutant strains of watermelon mosaic virus without specialized equipment in a short time. Moreover, the virus can be rapidly detected with the naked eye under natural light by using a high concentration of SYBR Green I. Therefore, the virus incurring huge damage to farms growing crops like pumpkin, melon, and watermelon can be detected early, thus the development of a more rapid and effective watermelon mosaic virus diagnosis system is expected.</p> |