METHODS OF MANUFACTURING BIOACTIVE GELS FROM EXTRACELLULAR MATRIX MATERIAL

摘要

The present invention is directed to methods of manufacturing bioactive gels from ECM material, i.e., gels which retain bioactivity, and can serve as scaffolds for preclinical and clinical tissue engineering and regenerative medicine approaches to tissue reconstruction. The manufacturing methods take advantage of a new recognition that bioactive gels from ECM material can be created by digesting particularized ECM material in an alkaline environment and neutralizing to provide bioactive gels.

主权项

1. A method of manufacturing a bioactive gel from an extracellular matrix material comprising:
a) providing an extracellular matrix material derived from a mammalian tissue and comprising bioactive components of the extracellular matrix material arranged and in quantities found in the tissue in its native form; b) particularizing the extracellular matrix material of a); c) solubilizing the particularized extracellular matrix material of b) in an alkaline solution; d) neutralizing the solution of step c) by the addition of equimolar concentrations of an acid.