A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.
申请公布号
WO2013158982(A1)
申请公布日期
2013.10.24
申请号
WO2013US37352
申请日期
2013.04.19
申请人
LIFE TECHNOLOGIES CORPORATION
发明人
CHEN, CAIFU;MCFARLAND, CASEY, R.;KEYS, JR., DAVID, N.