发明名称 |
Method for Columbidae gender identification, nucleotide sequence for Columbidae gender and nucleotide primer pair for Columbidae gender |
摘要 |
The invention provides a method for Columbidae gender identification including: providing a DNA sample of a bird belonging to the Columbidae family; performing a polymerase chain reaction to the DNA sample with a first primer pair of a first primer designed within the region of the SEQ ID. NO.: 9 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof, and a second primer pair of a second primer designed within the region of the SEQ ID NO.: 10 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof; and determining gender by performing a melting curve analysis to a product from the polymerase chain reaction, wherein the result showing two peaks indicate a female gender and showing one peak indicate a male gender.
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申请公布号 |
US8420316(B2) |
申请公布日期 |
2013.04.16 |
申请号 |
US20100854864 |
申请日期 |
2010.08.11 |
申请人 |
CHANG HSUEH-WEI;CHENG CHIEN-CHUNG;SU YING-FANG;HUNG YU-CHEN;KAOHSIUNG MEDICAL UNIVERSITY |
发明人 |
CHANG HSUEH-WEI;CHENG CHIEN-CHUNG;SU YING-FANG;HUNG YU-CHEN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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