METHOD FOR DETECTING NUCLEIC ACIDS BY PROMOTING BRANCHED DNA COMPLEX FORMATION

摘要

DISCLOSED IS A METHOD FOR DETECTING NUCLEIC ACIDS BY PROMOTING BRANCHED DNA COMPLEX FORMATION. THE TARGET NUCLEIC ACID DETECTION SIGNAL AND SENSITIVITY CAN BE DRAMATICALLY INCREASED BY PROMOTING SELF ASSEMBLY OF BRANCHED DNA BETWEEN A PLURALITY OF AMPLIFIED DNA TARGETS AND A SINGLE-CHAIN OLIGONUCLEOTIDE PROBE, BY MEANS OF THE INTEGRATED IMPLEMENTATION OF PCR, THERMAL DENATURATION AND HYBRIDIZATION IN A SINGLE REACTION MIXTURE.