SELECTION OF IMPROVED MICROBIAL STRAINS FOR PRODUCTION OF SUCCINIC ACID FROM GLYCEROL

摘要

Provided are methods for generating a microorganism with improved ability to convert glycerol to succinic acid. These methods comprise combining a microorganism with ability to convert glycerol to succinic acid and a medium containing glycerol to produce a fermentation mixture; allowing fermentation to occur such that succinic acid is produced; and assaying for an indication of glycerol metabolism. These latter two steps may be repeated as necessary. Also provided are microorganisms generated in this manner, particularly bacteria deposited at the NCIMB on 13 th April 2011 with the Accession Number NCIMB 41825. A method of producing succinic acid from glycerol is also provided, this method comprising: mixing a microorganism according to claim of the invention with a medium comprising glycerol to produce a fermentation mixture; and incubating the fermentation mixture, under conditions that promote fermentation to produce succinic acid, until succinic acid is produced. Succinic acid produced by such methods is also provided.