| 摘要 |
FIELD: medicine. ^ SUBSTANCE: what is presented is a method for producing a polyclonal antiserum which is immunospecifically bound to a biologically active parathyroid hormone (PTH) in a part of three or four N-terminal amino acid residues of (1-84) PTH. The first peptide antigen is introduced in a host animal other than a human. A titre of produced antibodies is monitored. Antiserum is extracted. At least one antibody is separated and recovered from antiserum by affine chromatography with using the second peptide antigen. It is followed by removing at least one antibody possessing specificity with respect to the peptide antigen specified in a group consisting of (4-34) PTH, (5-34) PTH, (4-84) PTH and (5-84) PTH by affine chromatography with using the third peptide antigen specified in a group consisting of (4-34) PTH, (5-34) PTH, (4-84) PTH and (5-84) PTH respectively. There is recovered polyclonal serum possessing binding affinity with respect to no more than the first three or four amino acid residues of the N-terminal part of PTH and possessing no affinity with respect to any amino acid residues in the fifth amino acid residue or after the fifth amino acid residue of the N-terminal of PTH. ^ EFFECT: determining the level of biologically active intact PTH in serum, plasma or cell culture medium. ^ 6 cl, 4 dwg |
