摘要 |
PURPOSE: A method for saccharification of cellulose using a strain is provided to ferment food waste and to manufacture low calorie food. CONSTITUTION: A Nectria cinnabarina KMJ523(KACC 93065P) produces cellulase. A method for preparing cellulase comprises a step of culturing the Nectria cinnabarina strains; and a step of isolating cellulase from the culture of Nectria cinnabarina. A medium for producing cellulase contains 20-30 g/L of avicel, 7-9 g/L of tryptone, 2-4 g/L of magnesium sulfate, 4-7 g/L of monobasic monopotassium phosphate, 4-7 g/L of dipotassium phosphate, and 2-3 g/L of inositol. The saccharification of cellulose is performed using Nectria cinnabarina strain, culture product of the strain, or celluloase from cellulose source. The cellulose source is rice bran, corn hull, corn, corn cop, sugarcane bagasse, or rice husk. |