发明名称 |
Method for single oxygen atom incorporation into digested peptides using peptidases |
摘要 |
Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or two 18O atoms. The outcome is accurate quantification of isotopically labeled peptides.
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申请公布号 |
US7476546(B2) |
申请公布日期 |
2009.01.13 |
申请号 |
US20050273806 |
申请日期 |
2005.11.15 |
申请人 |
UNIVERSITY OF NORTH DAKOTA |
发明人 |
MIYAGI MASARU;SEKHAR K. C. |
分类号 |
G01N1/00;C12P21/06 |
主分类号 |
G01N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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