| 摘要 |
PROBLEM TO BE SOLVED: To provide a method using O<SP>6</SP>-alkylguanine-DNA alkyltransferases (AGT) and for transferring a label from a substrate to a fusion protein comprising the AGT. SOLUTION: The detection and/or manipulating of the fusion protein, both in vitro and in vivo, are allowed by attaching molecules to the fusion proteins which molecules introduce a new physical or chemical property to the fusion protein. Examples of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilisation of the fusion protein. COPYRIGHT: (C)2008,JPO&INPIT
|
