| 摘要 |
The invention relates to a microorganism as a carrier of nucleotide seque nces coding for antigens and protein toxins comprising the following compone nts: (I) at least one nucleotide sequence coding for at least one complete o r partial antigen of at least one wild-type or mutated protein; and (II) at least one nucleotide sequence coding for at least one protein toxin and/or a t least one protein toxin subunit; and (III) a) at least one nucleotide sequ ence coding for at least one transport system which enables the expression o f the expression products of component (I) and component (II) on the outer s urface of the microorganism and/or enables the secretion of the expression p roducts of component (I) and component (II); and/or coding for at least one signal sequence which enables the secretion of the expression products of co mponent (I) and component (II); and/or (III) b) optionally, at least one nuc leotide sequence coding for at least one protein for lysing the microorganis m in the cytosol of mammalian cells and for intracellularly releasing plasmi ds or expression vectors, which are contained in the lysed microorganism; an d (IV) at least one nucleotide sequence for at least one activation sequence for the expression of one or more of components (I) to (III), wherein said activation sequence can be activated in the microorganism and/or is tissue c ell-specific, tumor cell-specific, macrophage-specific, dendrite-specific, l ymphocyte-specific, function-specific or non-cell-specific"; wherein any of components (I) to (IV) can be present either once or several times and eithe r identical or different. Also disclosed are a process of manufacturing ther eof, corresponding plasmids or expression vectors and uses of the microorgan ism as a medicament.
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