发明名称 |
METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM |
摘要 |
The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like. |
申请公布号 |
WO2007078127(B1) |
申请公布日期 |
2007.12.27 |
申请号 |
WO2006KR05886 |
申请日期 |
2006.12.29 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY;LEE, SANG YUP;PARK, TAE JUNG;HEO, NAM SU |
发明人 |
LEE, SANG YUP;PARK, TAE JUNG;HEO, NAM SU |
分类号 |
C12N15/64;C12N15/63;C12N15/70 |
主分类号 |
C12N15/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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