pETTvDAO2 RECOMBINANT PLASMID PROVIDING SYNTHESIS OF YEAST Trigonopsis variabilis D-AMINO ACID OXYDASE (DAO) IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C41(DE3)/pETTvDAO2 AS PRODUCER OF DAO

摘要

FIELD: biology, in particular gene engineering, microbiological industry, production of new beta-lactam antibiotics. ^ SUBSTANCE: constructed is recombinant plasmid DNA pETTvDAO2 providing high level of DNA fragment which encodes D-amino acid oxydase of Trigonopsis variabilis yeast in Escherichia coli cells. As a result of transformation of E.coli strain with disclosed recombinant plasmid and selection of transformed clones new recombinant Escherichia coli strain C41(DE3)/pETTvDAO2 as producer D-amino acid oxydase is obtained. ^ EFFECT: improved method for producing of cephalosporin antibiotics. ^ 2 cl, 4 dwg, 2 tbl, 7 ex