发明名称 |
Length determination of nucleic acid repeat sequences by discontinuous primer extension |
摘要 |
Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.
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申请公布号 |
US7294464(B2) |
申请公布日期 |
2007.11.13 |
申请号 |
US20040892403 |
申请日期 |
2004.07.14 |
申请人 |
APPLERA CORPORATION |
发明人 |
LIVAK KENNETH J.;LOWE ADAM L.;BLASBAND ANDREW J. |
分类号 |
C12N15/09;C12Q1/68;C07H21/04;C12P19/34;G01N33/53;G01N33/566 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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