MOLECULAR METHOD FOR THE GENETIC POPULATION ANALYSIS AND PEDIGREE ANALYSIS OF GILTHEAD SEABREAM (SPARUS AURATA) AND CORRESPONDING KIT

摘要

The invention relates to a molecular method for the genetic population analysis and pedigree analysis of gilthead seabream (Sparus aurata) and to the corresponding kit (Sparus aurata genotyping and paternity tool kit). More specifically, the invention relates to a molecular tool which is based on microsatellite markers, consisting of coamplification by polymerase chain reaction and analysis of 10 loci, thereby enabling the rapid and reliable provision of sufficient data relating to an individual or group of individuals in order to perform population studies and establish kinship or pedigree relationships with great precision. Said information can be used to control and even improve the characteristics of gilthead seabream broodstocks. In addition, the above protocol is suitable for the detection and automatic analysis used in applied biosystem (AB) sequencing equipment. The invention also relates to a kit with three possible formats, which can be used to standardise and automate the method in a simple and fully reproducible manner.