NOVEL SELECTION SYSTEM

摘要

The present invention relates to a method of producing a recombinant protein
comprising using a selection method other than antibiotics. In particular, it
relates to a stable host/vector system based on the pyrC gene complementation
designed to produce high level of heterologous recombinant protein in
Escherichia coli. The expression system of the present invention allows rapid
selection of plasmid containing cells during the cloning phases and lead to
high protein expression during fermentation. This system has a strong
selective efficiency, especially during the induction phase, leading to the
selection of an almost homogeneous and stable plasmid bearing cell population.
Moreover the productivity of the culture is to a large extent better than the
one based on antibiotic resistance. This elevated vector stability combined
with its high productivity fulfils the requirements for heterologous protein
production in Escherichia coli to an industrial level.